Equipment

New Brunswick BioFlo 4500 fermentor (20L capacity for bacterial and yeast cell growth)
The increase of recombinant protein expression in fermentor is largely due to the increase of biomass production under the optimized growth condition. Our BioFlo 4500 fermentor can provide either oxygen-enriched or anaerobic conditions. Some unique features of fermentor are:

  • Bacterial or yeast cultures
  • Up to 20L capacity
  • Precise temperature, agitation, and aeration control
  • pH monitoring and control
  • DO control and oxygen enrichment capability
  • Control of nutrient supplement

fermentor
Avestin EmulsiFlex C5 high pressure homogenizer
The high pressure homogenizer provides the most effective way to lyse cells using compressed nitrogen gas. Our Avestin EmulsiFlex C5 homogenizer can be used for cell rupture, preparation of liposomes, dispersion or emulsion of materials. The features of C5 are:

  1. Ideal for large scale cell rupture
  2. Adjustable pressure in the range of 500-30,000 psi
  3. Processing capacity 1-5L/hr

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GE Life Sciences AKTA Explorer100 and Purifier10 high performance liquid chromatography systems
AKTA High Performance Liquid Chromatography system is a versatile protein purification workstation which provides fast and robust choices to purify target proteins of interest. Some characteristic features of AKTA system are;

  1. Single platform for large and small scale protein/peptide purification
  2. Wide variety of commercial available resins and packed columns
  3. User friendly software to customize methods with flexible automatic and manual controls

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Beckman Coulter Proteome XL-I analytical ultracentrifuge
Analytical ultracentrifugation is a versatile and powerful method for study of the interactions of macromolecules. The Proteome XL-I analytical ultracentrifuge in our facility can characterize a variety of biophysical properties of macromolecules such as molecular weight, sedimentation coefficient, diffusion coefficient, equilibrium constant and stoichiometry. The abundant information provided by the analytical ultracentrifugation can assess the sample heterogeneity (aggregation and purify), molecular conformation (folded or unfolded), composition (assembled or unassembled), and thermodynamic properties of the interacting systems.

The Proteome XL-I analytical ultracentrifuge is configured with a scanning UV/Vis detection system and Rayleigh interference optics. The UV/Vis detection system provides sensitivity for low concentration samples and selectivity for optimizing detection based on the sample’s maximum absorbance. The Rayleigh interference optics provides the capability to measure the change in the refractive index resulting from the changes in sample compositions and concentration. The XL-I can collect data from either optic system that provides the broadest spectrum of data for characterization. In addition, we have both Anti-60 and Anti-50 rotors which can run up to 21 sample/reference pairs in a single run. This capability supports researchers to study macromolecules of interest in various experimental conditions.

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Beckman Coulter JLA 8.1000 centrifuge rotor
Thermo Savant SPD2010 Speedvac system